Virus:Article Title: Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies p-IkBa Cell Signaling Technology Cat#9246; RRID: AB_2267145 p-JNK Cell Signaling Technology Cat#9251; RRID: AB_331659 p-p38 Cell Signaling Technology Cat#9211; RRID: AB_331641 p-IKKa/b Cell Signaling Technology Cat#2697; RRID: AB_2079382 JNK Cell Signaling Technology Cat#9252; RRID: AB_2250373 p38 Cell Signaling Technology Cat#9212; RRID: AB_330713 IKKb Cell Signaling Technology Cat#2370; RRID: AB_2122154 MYC Cell Signaling Technology Cat#2276; RRID: AB_331783 FLAG Sigma-Aldrich Cat#F3165; RRID: AB_259529 actin Sigma-Aldrich Cat#A1978; RRID: AB_476692 HA Covance Cat#16B12; RRID: AB_10063630 TRAF6 Santa Cruz Cat#sc-7221; RRID: AB_793346 IBA1 abcam Cat#019-19741; RRID: AB_839504 DRD1 Hangzhou HuaAn Biotechnology CatET1703-45; RRID: AB_ 2815007 DRD2 Bioss Cat#bs-20730R; RRID: AB_ 2815008 DRD3 Affinity Cat#DF10212; RRID: AB_ 2815010 DRD4 Bioss Cat#bs-1746R; RRID: AB_ 10857578 DRD5 Santa Cruz Cat#sc-33661; RRID: AB_673639 DRD5 Proteintech Cat#20310-1-AP; RRID: AB_10699880 b-arrestin2 Santa Cruz Cat#sc-13140; RRID: AB_626701 b-arrestin2 Proteintech Cat#10171-1-AP; RRID: AB_10644158 IRDye 680RD-anti-mouse LI-COR Biosciences Cat#926-68070; RRID: AB_10956588 IRDye 800CW-anti-rabbit LI-COR Biosciences Cat#926-32211; RRID: AB_621843 Anti-mouse-HRP Invitrogen Cat#31430; RRID: AB_228307 Anti-rabbit-HRP Invitrogen Cat#31460; RRID: AB_228341 CD45-AF700 eBioscience Cat#56-0451-80; RRID: AB_891456 CD11b- FITC eBioscience Cat#11-0112-82; RRID: AB_464935 Bacterial and Virus Strains Staphylococcus aureus ATCC Cat#25923 Chemicals, Peptides, and Recombinant Proteins LPS EnzoLife Sciences Cat#ALX-581013L002 Pam3CSK4 Invivogen Cat#Tlrl-pms Poly(I:C) Invivogen Cat#PLC-37-07 RecFLA-ST Invivogen Cat#Tlrl-flic-10 CL097 Invivogen Cat#Tlrl-C97 ODN2395 Invivogen Cat#Tlrl-2395 SKF-38393 hydrobromide TOCRIS Cat#0922 Phenelzine sulfate salt Sigma-Aldrich Cat#P6777 3,5-Dinitrocatechol Sigma-Aldrich Cat#D131 A-68930 hydrochloride Sigma-Aldrich Cat#1534 Quinpirole Sigma-Aldrich Cat#096K4603 H89 CSNpharm Cat#CSN11132 KH7 MedChemExpress Cat#103194 (Continued on next page) e1 Molecular Cell 78, 1–15.e1–e6, April 2, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Trizol Life technologies Cat#15596018 Dopamine hydrochloride Sigma-Aldrich Cat#H8502 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 4’,6-Diamidino-2-phenylindoledihydrochloride (DAPI) Sigma-Aldrich Cat#D9542 Anti-Myc magnetic beads Biomake Cat#B26301 Critical Commercial Assays Mouse IL-6 DuoSet ELISA R&D Systems Cat#DY406 Mouse TNF-alpha DuoSet ELISA R&D Systems Cat#DY410 Mouse IL-12/IL-23 p40 Non Allele-specific DuoSet R&D Systems Cat#DY2398 Deposited Data RNA-seq NCBI Gene Expression Omnibus GSE 133316 Raw images of western blots and original image files Mendeley Data https://doi.org/10.17632/r68gw2wbwy.1 Experimental Models: Cell Lines Human:HEK293T ATCC Cat#CRL-11268 Mouse:Primary bone marrow-derived macrophages This paper N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory Stock No: 000664 Mouse: Drd1 / Dr. Jiawei Zhou, SIBS,China N/A Mouse: Drd2 / Dr. Jiawei Zhou, SIBS,China N/A Mouse:Arrb2 / Dr.
Recombinant:Article Title: Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies p-IkBa Cell Signaling Technology Cat#9246; RRID: AB_2267145 p-JNK Cell Signaling Technology Cat#9251; RRID: AB_331659 p-p38 Cell Signaling Technology Cat#9211; RRID: AB_331641 p-IKKa/b Cell Signaling Technology Cat#2697; RRID: AB_2079382 JNK Cell Signaling Technology Cat#9252; RRID: AB_2250373 p38 Cell Signaling Technology Cat#9212; RRID: AB_330713 IKKb Cell Signaling Technology Cat#2370; RRID: AB_2122154 MYC Cell Signaling Technology Cat#2276; RRID: AB_331783 FLAG Sigma-Aldrich Cat#F3165; RRID: AB_259529 actin Sigma-Aldrich Cat#A1978; RRID: AB_476692 HA Covance Cat#16B12; RRID: AB_10063630 TRAF6 Santa Cruz Cat#sc-7221; RRID: AB_793346 IBA1 abcam Cat#019-19741; RRID: AB_839504 DRD1 Hangzhou HuaAn Biotechnology CatET1703-45; RRID: AB_ 2815007 DRD2 Bioss Cat#bs-20730R; RRID: AB_ 2815008 DRD3 Affinity Cat#DF10212; RRID: AB_ 2815010 DRD4 Bioss Cat#bs-1746R; RRID: AB_ 10857578 DRD5 Santa Cruz Cat#sc-33661; RRID: AB_673639 DRD5 Proteintech Cat#20310-1-AP; RRID: AB_10699880 b-arrestin2 Santa Cruz Cat#sc-13140; RRID: AB_626701 b-arrestin2 Proteintech Cat#10171-1-AP; RRID: AB_10644158 IRDye 680RD-anti-mouse LI-COR Biosciences Cat#926-68070; RRID: AB_10956588 IRDye 800CW-anti-rabbit LI-COR Biosciences Cat#926-32211; RRID: AB_621843 Anti-mouse-HRP Invitrogen Cat#31430; RRID: AB_228307 Anti-rabbit-HRP Invitrogen Cat#31460; RRID: AB_228341 CD45-AF700 eBioscience Cat#56-0451-80; RRID: AB_891456 CD11b- FITC eBioscience Cat#11-0112-82; RRID: AB_464935 Bacterial and Virus Strains Staphylococcus aureus ATCC Cat#25923 Chemicals, Peptides, and Recombinant Proteins LPS EnzoLife Sciences Cat#ALX-581013L002 Pam3CSK4 Invivogen Cat#Tlrl-pms Poly(I:C) Invivogen Cat#PLC-37-07 RecFLA-ST Invivogen Cat#Tlrl-flic-10 CL097 Invivogen Cat#Tlrl-C97 ODN2395 Invivogen Cat#Tlrl-2395 SKF-38393 hydrobromide TOCRIS Cat#0922 Phenelzine sulfate salt Sigma-Aldrich Cat#P6777 3,5-Dinitrocatechol Sigma-Aldrich Cat#D131 A-68930 hydrochloride Sigma-Aldrich Cat#1534 Quinpirole Sigma-Aldrich Cat#096K4603 H89 CSNpharm Cat#CSN11132 KH7 MedChemExpress Cat#103194 (Continued on next page) e1 Molecular Cell 78, 1–15.e1–e6, April 2, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Trizol Life technologies Cat#15596018 Dopamine hydrochloride Sigma-Aldrich Cat#H8502 AceQ qPCR SYBR Green Master Mix Vazyme Cat#Q111-02 4’,6-Diamidino-2-phenylindoledihydrochloride (DAPI) Sigma-Aldrich Cat#D9542 Anti-Myc magnetic beads Biomake Cat#B26301 Critical Commercial Assays Mouse IL-6 DuoSet ELISA R&D Systems Cat#DY406 Mouse TNF-alpha DuoSet ELISA R&D Systems Cat#DY410 Mouse IL-12/IL-23 p40 Non Allele-specific DuoSet R&D Systems Cat#DY2398 Deposited Data RNA-seq NCBI Gene Expression Omnibus GSE 133316 Raw images of western blots and original image files Mendeley Data https://doi.org/10.17632/r68gw2wbwy.1 Experimental Models: Cell Lines Human:HEK293T ATCC Cat#CRL-11268 Mouse:Primary bone marrow-derived macrophages This paper N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory Stock No: 000664 Mouse: Drd1 / Dr. Jiawei Zhou, SIBS,China N/A Mouse: Drd2 / Dr. Jiawei Zhou, SIBS,China N/A Mouse:Arrb2 / Dr.
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